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Standard RNA Phosphoramidite
Product Breadth of Proligo Reagents
| RNA plays a pivotal role in biological systems due to its numerous function in the transfer and processing of genetic information. The unique properties of RNA have stimulated the development of a variety of applications in diagnostics and therapeutics, as well as in basic molecular biology research where RNA can be used as: |
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Catalytic agents (ribozymes) |
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Affinity ligands (aptamers) |
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Agents to induce gene silencing (RNA interference) |
| Today, RNA interference (RNAi) has become a popular tool for the sequence-specific inhibition of gene expression and can be used in target validation and other drug development techniques. The most convenient method to provide sequence specific RNA oligonucleotides is chemical synthesis on a solid support with RNA phosphoramidites and RNA CPG, analogous to DNA synthesis. |
Key features of Standard RNA Amidites
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Industry standard 2'-TBDMS protective group |
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Lot-to-lot consistent purity and performance |
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Compatible with deprotection methods based on
methylamine or AMA |
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Standard RNA Amidites provide excellent coupling
results when used together with ETT or BTT as activator;
best results are obtained with Activator 42™1
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Capping with standard acetic anhydride capping
reagent rather than with Fast Deprotection Cap A |
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Manufactured under a certified ISO 9001 quality system |
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| RNA Phosphoramidites |
| Compatible with Expedite™ and Polygen® Instruments |
| Product No. |
Description |
Unit |
| A211081-01 |
DMT-2'O-TBDMS-rA(bz) Amidite |
1 x 0.5g |
| C213081-01 |
DMT-2'O-TBDMS-rC(ac) Amidite |
1 x 0.5g |
| G211081-01 |
DMT-2'O-TBDMS-rG(ib) Amidite |
1 x 0.5g |
| U211081-01 |
DMT-2'O-TBDMS-rU Amidite |
1 x 0.5g |
| Compatible with ABI™ Instruments |
| Product No. |
Description |
Unit |
| A211031-01 |
DMT-2'O-TBDMS-rA(bz) Amidite |
1 x 0.5g |
| A211061-01 |
DMT-2'O-TBDMS-rA(bz) Amidite |
1 x 1 g |
| C213031-01 |
DMT-2'O-TBDMS-rC(ac) Amidite |
1 x 0.5g |
| C213061-01 |
DMT-2'O-TBDMS-rC(ac) Amidite |
1 x 1 g |
| G211031-01 |
DMT-2'O-TBDMS-rG(ib) Amidite |
1 x 0.5g |
| G211061-01 |
DMT-2'O-TBDMS-rG(ib) Amidite |
1 x 1 g |
| U211031-01 |
DMT-2'O-TBDMS-rU Amidite |
1 x 0.5g |
| U211061-01 |
DMT-2'O-TBDMS-rU Amidite |
1 x 1 g |
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Other quantities and packaging are available upon request. |
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| 1Activator 42 = 5-(3,5-Bis(trifluoromethyl)phenyl)-1H-tetrazole, a very powerful activator for RNA- and DNA phosphoramidites, commercially available through SAFC Proligo Reagents. |
SAFC and Proligo are registered trademarks of Sigma-Aldrich Co. LLC.
©2012 Sigma-Aldrich Co. LLC. All rights reserved. Reproduction forbidden without permission.
Other trademarks are the property of their respective owners.
To order or speak with a Customer Service Representative, contact your local SAFC office.
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