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ssH-Linker
Product Breadth of Proligo Reagents
Phosphoramidite technology is ideally suited for the modification of synthetic oligonucleotides with covalently attached linkers. SAFC Proligo Reagents™ provides ready to use linkers for DNA/RNA synthesis machines as β-cyanoethyl phosphoramidites.
Amino linkers are particularly suitable for the conjugation of biotin, fluorescent dyes or other modifiers and reporter groups to the 5' end of oligonucleotides, or for the attachment of oligonucleotides to microarrays.
In addition to established linkers MMT*-protected amino linker : ssH-Linker |
| ssH-Linker |
| Compatible with Expedite™ and Polygen® Instruments |
| Product No. |
Description |
Quantity |
| M010982-01 |
ssH-Linker |
1 x 0.25g |
| Compatible with ABI® Instruments |
| Product No. |
Description |
Unit |
| M010932-01 |
ssH-Linker |
1 x 0.25g |
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Advantages over conventional amino linkers:
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Deprotection of the amino-group under exceptionally mild conditions which avoid depurination side reaction |
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Very high labelling efficiency |
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Better trityl-on purification efficiency |
General features:
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Excellent coupling efficiency |
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High solubility in acetonitrile |
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Coupling through standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites |
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Used with standard deblock-, activator-, oxidizer- and capping-solutions |
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Features a lipophilic MMT-group to support the purification of the oligonucleotide synthesis product with reversed-phase cartridges or by HPLC |
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Permits measurement of the coupling efficiency through colorimetric MMT-assays |
* monomethoxytrityl
ssH-Linker is covered by the patents / patent applications JP 2005-217026 and PCT/JP2005/021135.
SAFC and Proligo are registered trademarks of Sigma-Aldrich Co. LLC.
©2012 Sigma-Aldrich Co. LLC. All rights reserved. Reproduction forbidden without permission.
Other trademarks are the property of their respective owners.
To order or speak with a Customer Service Representative, contact your local SAFC office.
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