| Key Features of 5’-Phosphorylated Oligonucleotides |
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Enables enzymatic ligation to the 3'- terminus of nucleic acids |
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Offers protection against exonucleolytic degradation |
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Provides oligonucleotide conjugation through activation with coupling reagents such as EDC |
These features enable 5’-phosphorylated oligonucleotides to be used in applications such as molecular cloning and gene construction; ligase chain reaction; template directed ligation; oligonucleotide labeling with reporter groups, haptens and other modifiers; and oligonucleotide stabilization.
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| Key Features of Phosphate-ON Phosphoramidite |
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Fully compatible with standard phosphoramidite reagents and synthesis conditions |
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Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotide can be cleaved from the support and deprotected in concentrated ammonia at 55°C in only 2 hours |
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Comprises a DMT-group for trityl monitoring |
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Dissolves easily in acetonitrile to a 0.1M solution |
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Powder format enables dissolution to be easily monitored and facilitates product handling |
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High coupling efficiency of ≥ 98.0% under standard DNA phosphoramidite coupling conditions |
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Cleavage of both phosphate protective groups through a β-elimination process |
