Phosphate-ON phosphoramidite is a chemical phosphorylation reagent that can be employed to introduce a phosphate group at the 5’ terminus of an oligonucleotide. Chemical phosphorylation is a cost effective alternative to enzymatic phosphorylation methods, allowing introduction of terminal phosphate groups in high yields, at any desired scale.

SAFC Proligo Reagents’ offers Phosphate-ON phosphoramidite in powder form, enabling the dissolution of the reagent in acetonitrile to be easily monitored. The application of the reagent is fully compatible with standard cleavage and deprotection procedures at the end of oligonucleotide synthesis. Phosphate-ON phosphoramidite from Proligo Reagents comprises a thymidine nucleoside, which is removed from the oligonucleotide during the cleavage and deprotection process.

Download Literature:
•	User Instructions Phosphate-ON Phosphoramidite (668k pdf)
View Structure:
•	Phosphate-ON Phosphoramidite

Key Features of 5’-Phosphorylated Oligonucleotides
	Enables enzymatic ligation to the 3'- terminus of nucleic acids
	Offers protection against exonucleolytic degradation
	Provides oligonucleotide conjugation through activation with coupling reagents such as EDC
These features enable 5’-phosphorylated oligonucleotides to be used in applications such as molecular cloning and gene construction; ligase chain reaction; template directed ligation; oligonucleotide labeling with reporter groups, haptens and other modifiers; and oligonucleotide stabilization.
Key Features of Phosphate-ON Phosphoramidite
	Fully compatible with standard phosphoramidite reagents and synthesis conditions
	Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotide can be cleaved from the support and deprotected in concentrated ammonia at 55°C in only 2 hours
	Comprises a DMT-group for trityl monitoring
	Dissolves easily in acetonitrile to a 0.1M solution
	Powder format enables dissolution to be easily monitored and facilitates product handling
	High coupling efficiency of ≥ 98.0% under standard DNA phosphoramidite coupling conditions
	Cleavage of both phosphate protective groups through a β-elimination process

Phosphate on Phosphoramidite
Compatible with Expedite™ and Polygen® Instruments
Product No.	Description	Unit
M010782-01	Phosphate-ON amidite
Compatible with ABI™ Instruments
M010732-01	Phosphate-ON amidite

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