Fluorescein phosphoramidite is composed of a protected fluorescein molecule linked to a ß-cyanoethylphosphoramidite. The fluorescein derivative consists of to isomers derived form 5- and 6- carboxy fluorescein. As a result, two peaks will be seen on a RP-HPLC chromatogram of the synthesis product.

Key Features of the Fluorescein Phosphoramidite
	Readily soluble in acetonitrile
	Couples to the 5’ end of the oligonucleotide using standard synthesis protocols
	Protecting groups on the fluorescein moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia
	A detritylation step is not required since the fluorescein phosphoramidite does not contain a dimethoxytrityl (DMT-) group
	The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required
	The fluorescein moiety provides a purification handle in RP-HPLC purification

Download Literature:
•	User Instructions Fluorescein Phosphoramidite (656k pdf)
View Structure:
•	Fluorescein-ß- Cyanoethyl phosphoramidite

Labels and Modifications - Fluorescein Phosphoramidite
Compatible with Expedite™ and Polygen® Instruments
Product No.	Description	Unit
M010181-01	Fluorescein amidite	1 x 0.1g
Compatible with ABI™ Instruments
M010131-01	Fluorescein amidite	1 x 0.1g
Custom Products	Phosphoramidites of non-catalogue linkers, reporters or modifiers are offered as custom synthesis products. Please enquire.

SAFC and Proligo are registered trademarks of Sigma-Aldrich Co. LLC.
©2012 Sigma-Aldrich Co. LLC. All rights reserved. Reproduction forbidden without permission.
Other trademarks are the property of their respective owners.

To order or speak with a Customer Service Representative, contact your local SAFC office.