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DMT-2'Fluoro Phosphoramidites
| Product Breadth of Proligo Reagents |
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| Product Breadth of Proligo Reagents |
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| 2'Fluoro Phosphoramidites are used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance. |
| Can be employed together with DNA or RNA phophoramidites |
| All four bases are available: |
• DMT-2'Fluoro-dA(bz) Phosphoramidite
• DMT-2'Fluoro-dC(ac) Phosphoramidite
• DMT-2'Fluoro-dG(ib) Phosphoramidite
• DMT-2'Fluoro-dU Phosphoramidite |
| Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C |
| Synthesis of 2'Fluoro oligonucleotides is similar to standard DNA synthesis, but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers) |
| Lot to lot consistent purity and performance |
| Manufactured under a certified ISO 9000 quality system since 1993 |
| Analytical Specifications of 2'Fluoro Amidites |
| Test |
2'F-dA(bz) |
2'F-dC(ac) |
2'F-dG(ib) |
2'F-dU |
| Reverse Phase HPLC |
≥98.0% |
≥98.0% |
≥98.0% |
≥98.0% |
| 31P-NMR |
≥98% |
≥99% |
≥98% |
≥99% |
| Ordering Information |
| DMT-2'Fluoro Phosphoramidites |
| Compatible with Expedite™ and Polygen® Instruments |
| Product No. |
Description |
Unit |
| A211281-01 |
DMT-2'Fluoro-dA(bz) Amidite |
1 x 0.25g |
| C213281-01 |
DMT-2'Fluoro-dC(ac) Amidite |
1 x 0.25g |
| G211281-01 |
DMT-2'Fluoro-dG(ib) Amidite |
1 x 0.25g |
| U211281-01 |
DMT-2'Fluoro-dU Amidite |
1 x 0.25g |
| Compatible with ABI® Instruments |
| A211231-01 |
DMT-2'Fluoro-dA(bz) Amidite |
1 x 0.25g |
| C213231-01 |
DMT-2'Fluoro-dC(ac) Amidite |
1 x 0.25g |
| G211231-01 |
DMT-2'Fluoro-dG(ib) Amidite |
1 x 0.25g |
| U211231-01 |
DMT-2'Fluoro-dU Amidite |
1 x 0.25g |
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