Biotin-labeled oligonucleotides have become extremely popular in DNA capture and detection applications, due to their very high sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotin-based detection methods are widely used by researchers. Biotin labeling is compatible with PCR and most hybridization techniques.

Key Features of the Biotin Phosphoramidite
		Behaves like any standard amidite on the DNA synthesizer
		Readily soluble in acetonitrile
		Biotin compound has a dimethozytrityl (DMT) — group on the ring structure, which allows coupling-yield determination and trityl-selective purification
		A long, water-compatible linker arm ensures maximum sensitivity
		The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required
		Provides a coupling efficiency of 95%

Download Literature:
User Instructions
Biotin Phosphoramidite
(670 Kb PDF)

View Structure:
DMT-Biotin-β-Cyanoethy-lphosphoramidite

Labels and Modifications - Biotin Phosphoramidite
Compatible with Expedite™ and Polygen® Instruments
Product No.	Description	Unit
M010381-01	Biotin amidite	0.1g
M010382-01	Biotin amidite	0.25 g
Compatible with ABI™ Instruments
M010331-01	Biotin amidite	0.1g
M010332-01	Biotin amidite	0.25 g
Custom Products	Phosphoramidites of non-catalogue linkers, reporters or modifiers are offered as custom synthesis products. Please enquire.

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