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Amino-ON CPG
Product Breadth of Proligo Reagents
| Analytical Parameters of CPG |
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CPG (UHL) |
CPG 500Å |
CPG 1000Å |
| Pore size (nm) |
33 |
50 |
100 |
| Pore volume (ml/g) |
1.3 |
1.3 |
1.3 |
| Grain size (µm) |
100 - 180 |
100 – 180 |
100 - 180 |
| Surface (m2/g) |
~100 |
~60 |
~30 |
| Amino-ON CPG can be employed to conjugate biotin, fluorescein or other modifiers and reporter groups to the 3'-end of oligonucleotides or to attach oligonucleotides to surfaces. Amino-ON CPG from SAFC Proligo Reagents combines the advanced features of cleavage and deprotection under mild conditions, and maintenance of the integrity of the 3'-modification with high synthesis yields. |
| Key Features of 3'-Amine Oligonucleotides |
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Selective conjugation to reporter groups, purification handles or other modifiers |
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3'-end attachment to electrophilic surfaces |
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Offers protection against exonucleolytic degradation |
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These features enable 3'-amine oligonucleotides to be used in applications such as 3'-labeling with base sensitive dyes; 3'-biotin modifications; dual-labeled probes (e.g. TaqMan® probes and Molecular beacons); and microarray spotting.
| Key Features of Amino-ON CPG |
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Introduces a primary amino group attached to a C6-linker |
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Fully compatible with standard phosphoramidite reagents and synthesis conditions |
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Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides |
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Can be used with DNA, RNA and Locked Nucleic Acid® (LNA®); 5'-oligonucleotide modifications such as fluorescein or biotin; and with the synthesis of thioated DNA oligonucleotides |
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Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotide can be cleaved from the support and deprotected in concentrated ammonia at 55°C in 2 hours |
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Provides all the advantages of homogeneous pore size porous glass supports |
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Standard pore size of 500Å |
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Loading of 30-40 µmol/g |
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Coupling performance of ≥ 99.0% stepwise efficiency |
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DMT-protected in the same way as standard nucleoside-loaded CPG |
| Amino on CPG |
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500 Å, extent of labeling: 30‑40 μmol/g |
| M020101-01 |
Amino-ON CPG |
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| M020111-01 |
Amino-ON CPG |
1 x 10 g |
SAFC and Proligo are registered trademarks of Sigma-Aldrich Co. LLC.
©2012 Sigma-Aldrich Co. LLC. All rights reserved. Reproduction forbidden without permission.
Other trademarks are the property of their respective owners.
To order or speak with a Customer Service Representative, contact your local SAFC office.
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