| Amino Linkers can be employed to conjugate biotin, fluorescein or other modifiers and reporter groups to the 5’end of oligonucleotides, or to attach oligonucleotides to surfaces. SAFC Proligo Reagents offers monomethoxytrityl-protected amino linkers as well as trifluoroacetyl-protected amino linkers. |
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| Monomethoxytrityl (MMT)-protected amino linker |
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The MMT-group can be cleaved on the synthesis instrument with acidic deblock solution to enable on-support labeling protocols. Alternatively, the MMT-amino linker can be attached in the trityl-on mode of the instrument to provide purification handle similar to the DMT-group of the conventional oligonucleotides. The MMT-group is then removed with aqueous acid after purification with either RP-HPLC or a purification cartridge. |
| Trifluoroacetyl (TFA)-protected amino linker |
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The base-labile TFA-group is easily removed with concentrated ammonia during the cleavage and deprotection step. Additional deprotection steps are not necessary. |
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| Key features of Amino Linkers |
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Completely soluble in acetonitrile |
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Proligo Reagents offers base-labile or acid-labile protecting groups on the amino linker, depending upon the application |
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Amino linker products are coupled with standard synthesis protocols, identical to the coupling pf DNA monomer phosphoramidites |
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No change in auxiliary synthesis reagents is required |
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The MMT-amino linker features a lipophilic group which aids in purification of the modified oligonucleotide after synthesis |
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The acid-labile MMT-group permits the colorimetric, determination of the coupling efficiency |
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It is recommended to deprotect MMT-amino linker oligonucleotides in concentrated ammonia at a lower temperature e.g. at 40°C for 24 hours |
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The MMT-group can be removed in aqueous solution with 80% acetic acid at room temperature for 24 hours |
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TFA-amino linker is completely deprotected with concentrated ammonia. Additional deprotection steps are not necessary |
