| Activator 42 introduces unprecedented activation power to phosphoramidite coupling reactions. This novel activator provides unique opportunities to speed up oligonucleotide synthesis and to improve on product yield. |
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| Key features of Activator 42 |
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The highest activation efficiency in the industry compared to other activators |
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Promotes ultrafast DNA couplings (coupling time less than 15 seconds) |
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Promotes rapid RNA couplings (coupling time 6 minutes) |
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Excellent solubility in acetonitrile (greater than 0.9M) |
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Not hygroscopic |
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Safe to handle (no explosive properties) |
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Very high overall yield in DNA and RNA synthesis |
| Solid phase oligonucleotide synthesis with phosphoramidites consists of a sequence of three consecutive reaction steps which are repeated in a cyclical manner, deblocking, coupling and oxidation. |
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Whereas deblocking and oxidation reactions proceed with nearly quantitative yields in state of the art oligonucleotide synthesis, the coupling reactions, although high yielding, determine the overall efficiency of the process. Coupling yields are driven by a variety of factors, including amidite and solid support quality, water content of the coupling medium and the choice of activator molecule employed. An ideal activator should promote the coupling of standard DNA amidites as well as the coupling of more sterically demanding amidites (e.g. 2’O-TBDMS-RNA amidites) in highly efficient manner. The activator should have excellent solubility in the coupling medium (i.e. acetonitrile) in order to avoid undesired precipitation or crystallisation, should not be hygroscopic, should be safe to handle and should not lead to side reactions in oligonucleotide synthesis. These desired properties can be attributed by SAFC Proligo Reagents to a new powerful activator molecule which gives superior performance compared to the other commercial activators such as 5-ethylthio-1 H-tetrazole (ETT), 5-benzylthio-1H-tetrazole (BTT) and 4,5-dicyanoimidazole (DCI).
Activation efficiency for 2’O-TBDMS-RNA amidites Activator 42 > ETT = BTT
Activation efficiency for standard DNA amidites Activator 42 > DCI |
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