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6-FAM
Product Breadth of Proligo Reagents
Download Literature: 6-FAM (414 Kb PDF)
| 6-FAM as a common fluorescent derivatization reagent for covalent labelling of synthetic oligonucleotides. |
| 6-FAM consists of one isomer derived from 6-carboxy fluorescein |
| Couples to the 5'-end of the oligonucleotide using standard synthesis protocols |
| Protecting groups on the fluorescein moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia |
| A detritylation step is not required since the fluorescein phosphoramidite does not contain a DMT-group |
| The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia—standard procedures can be used if additional purification is required |
| Lot-to-lot consistent purity and performance |
| Manufactured under a certified ISO 9000 quality system since 1993 |
| Analytical Specifications of 6-FAM |
| Test |
6-FAM |
| HPLC Purity |
≥97.0% |
| 31P-NMR |
≥97.0% |
| Ordering Information |
| 6-FAM |
| Compatible with Expedite™ and Polygen® Instruments |
| Product No. |
Description |
Unit |
| X010181-01 |
6-FAM |
1 x 0.1g |
| Compatible with ABI® Instruments |
| X010131-01 |
6-FAM |
1 x 0.1g |
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