| 2’O-Methyl RNA is a nucleic acid analogue that is characterized by the exceptional hybridization properties that it imparts with complimentary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids. |
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| The unique combination of properties of 2’O-Methyl RNA has found widespread use in the fields of: |
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Diagnostic probes |
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Aptamer and ribozyme development |
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Mixed 2’O-Methyl-RNA/DNA antisense molecules |
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2’O-Methyl RNA nucleoside can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.
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| Key features of 2’O-Methyl RNA Phosphoramidites |
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High yield of oligonucleotides |
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Compatible with DNA synthesis |
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Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides |
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Recommended deprotection conditions are 8 hours at 55°C using concentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65°C |
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Purification and other downstream processing of fully modified 2’O-Methyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation |
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Synthesis of 2’O-Methyl RNA oligonucleotides is similar to standard DNA synthesis, but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers) |
