Biocides & Preservatives

Diagnostics Product Group

ProClin® Preservatives:

	Key features of ProClin Preservatives
	Mechanism of Action
	ProClin Evaluation Kits & Packaging
	ProClin Features

	ProClin Preservatives Product List
	Sodium Azide Product List
	ProClin Product Information

ProClin preservatives are an excellent choice for replacing thimerosal, sodium azide and gentamicin preservatives. In addition they are highly effective, broad-spectrum biocides for controlling microorganisms in reagents and products intended for in vitro diagnostics use. At concentrations as low as 0.02%, these preservatives eradicate bacteria, fungi and yeast for extended periods of time.

View our recent article in Specialty Chemicals Magazine discussing the critical role of IVD preservatives from an environmental perspective.

Key features of ProClin preservatives
	Compatibility with key enzymes and assay indicators
	Will not inhibit antibody binding
	Easy-to-use water-soluble liquid, effective at very low concentrations
	Excellent stability over a wide pH range
	Level of active ingredients assayable by HPLC (Inquire for a HPLC standard)
	At recommended usage levels, ProClin presents no health hazards, toxological problems or disposal issues

Mechanism of Action
The active components of ProClin are three isothiazolones, 2-Methyl-4-isothiazolin-3-one, 5-Chloro-2-methyl-4-isothiazolin-3-one and 1,2-Benzisothiazolin-3-one.

Within minutes of contacting a microorganism, the active components penetrate the cell wall and inhibit specific enzymes (at the points noted in red in the Krebs Cycle).

Growth, macromolecule synthesis and respiration are inhibited, ultimately leading to cell death.

ProClin Evaluation Kits
ProClin Evaluation Kits are available for determining the suitability of ProClin products in new product development. Each evaluation kit contains three different manufactured lots of ProClin for testing performance consistency from lot to lot.

To determine the appropriate ProClin product for a specific application, ProClin variety kits are also available. Each kit contains 5 mL of Proclin 150, 200, 300 and 950.

ProClin Evaluation Kits
33360-U	ProClin Reference Standard - 1 mL ampule (1.5% A.I. solution)
48121	ProClin 150 – 3 x 5 mL (3 lot numbers)
49381-U	ProClin 150 – 3 x 400 mL (3 lot numbers)
48911-U	ProClin 300 – 3 x 5 mL (3 lot numbers)
48119-U	ProClin Variety Kit – 5 mL each of ProClin 150, 200, 300 & 950 as an aid to select the appropriate ProClin product for a specific application

ProClin Features

Features	ProClin B-119	ProClin 150	ProClin 200	ProClin 300	ProClin 950
Active (A.I.)	BIT	CMIT/MIT	CMIT/MIT	CMIT/MIT	MIT
% total A.I.	18.8 – 19.9	1.5	1.5	3	9.5
Stabilizers	none	23 – 25% Mg salts	3% Mg & Cu salts	Alkyl Carboxylate	none
Matrix	Dipropylene Glycol (60%) Water (15%) Sodium Hydroxide (6%)	Water	Water	Propylene Glycol	Water
Working pH Range	4 – 12	2.5 – 8.5	2.5 – 8.5	2.5 – 8.5	2 – 12
Typical Dosage Levels (w/w)	0.05 – 0.15%	0.05 – 0.10%	0.05 – 0.10%	0.03 – 0.05%	0.05 – 0.10%
Specific Gravity	1.14	1.20	1.02	1.03	1.02
Shelf Life	2 years	4 years	18 months	3 years	3 years

ProClin Preservatives Product List
Description	Catalog No.
ProClin B-119 Preservative
50 mL bottle	40424-U
400 mL bottle	40425-U
ProClin 150 Preservative
50 mL bottle	49376-U
400 mL bottle	49377-U
3.6 L bottle	49378-U
15 L pail	49379-U
110 kg drum (91.7 L)	49380-U
ProClin 200 Preservative
50 mL bottle	48171-U
400 mL bottle	500380
3.6 L bottle	500399
15 L pail	500402
ProClin 300 Preservative
1 x 5 mL vial	48934-U
50 mL bottle	48912-U
400 mL bottle	48914-U
2.0 L bottle	48915-U
3.6 L bottle	48917-U
18 L pail	48918-U
110 kg drum (107.8 L)	48919-U
ProClin 950 Preservative
5 mL ampule	46885-U
50 mL bottle	46878-U
400 mL bottle	46879-U
3.6 L bottle	46883-U
17 L pail	46884-U

Sodium Azide Product List
Description	Catalog No.
SigmaUltra	S8032
ReagentPlus®, ≥99.5%	S2002
purum p.a., ≥99.0% (T)	71290